• Login
    View Item 
    •   DSpace Home
    • Centro Académico de Salud (CAS)
    • Hospital Universitario Austral -HUA-
    • Investigación Aplicada
    • View Item
    •   DSpace Home
    • Centro Académico de Salud (CAS)
    • Hospital Universitario Austral -HUA-
    • Investigación Aplicada
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Possible neuroprotective role of P2X2 in the retina of diabetic rats.

    Thumbnail
    View/Open
    IA - Gallo (4.775Mb)
    Date
    2018-04-12
    Author
    Mancini, Jorge.
    Ortiz, Gustavo.
    Potilinski, Constanza.
    Salica, Juan.
    Lopez, Emiliano S.
    Croxatto, Oscar.
    Gallo, Juan E.
    Metadata
    Show full item record
    Abstract
    Abstract Background: Purinergic receptors are expressed in diferent tissues including the retina. These receptors are involved in processes like cell growth, proliferation, activation and survival. ATP is the major activator of P2 receptors. In diabetes, there is a constant ATP production and this rise of ATP leads to a persistent activation of purinergic receptors. Antagonists of these receptors are used to evaluate their inhibition efects. Recently, the P2X2 has been reported to have a neuroprotective role. Methods: We carried out a study in groups of diabetic and non-diabetic rats (N=5) treated with intraperitoneal injections of PPADS, at 9 and 24 weeks of diabetes. Control group received only the bufer. Animals were euthanized at 34 weeks of diabetes or at a matching age. Rat retinas were analyzed with immunohistochemistry and western blot using antibodies against GFAP, P2X2, P2Y2 and VEGF-A. Results: Diabetic animals treated with PPADS disclosed a much more extended staining of VEGF-A than diabetics without treatment. A lower protein expression of VEGF-A was found at the retina of diabetic animals without treatment of purinergic antagonists compared to diabetics with the antagonist treatment. Inhibition of P2X2 receptor by PPADS decreases cell death in the diabetic rat retina. Conclusion: Results might be useful for better understanding the pathophysiology of diabetic retinopathy.
    URI
    https://riu.austral.edu.ar/handle/123456789/948
    Collections
    • Investigación Aplicada

    xmlui.dri2xhtml.structural.info-link
    Licencia Creative Commons
    xmlui.dri2xhtml.structural.contact-link1 - xmlui.dri2xhtml.structural.contact-link2
    xmlui.dri2xhtml.structural.info-link2
     

     


    xmlui.dri2xhtml.structural.info-link
    Licencia Creative Commons
    xmlui.dri2xhtml.structural.contact-link1 - xmlui.dri2xhtml.structural.contact-link2
    xmlui.dri2xhtml.structural.info-link2