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dc.contributor.authorGimeno, Maria.
dc.contributor.authorFuertes, Florencia.
dc.contributor.authorBarcala, Andres.
dc.contributor.authorAttorressi, Alejandra.
dc.contributor.authorCucchiani, Rodolfo.
dc.contributor.authorCorrales, Luis.
dc.contributor.authorOliveira, Talita.
dc.date.accessioned2020-02-18T19:01:46Z
dc.date.available2020-02-18T19:01:46Z
dc.date.issued2017-01-01
dc.identifier.citationStem Cells Transl Med. 2017 Jan;6(1):161-173en_US
dc.identifier.issn2157-6564
dc.identifier.urihttps://riu.austral.edu.ar/handle/123456789/814
dc.description.abstractAdult mesenchymal stromal cell-based interventions have shown promising results in a broad range of diseases. However, their use has faced limited effectiveness owing to the low survival rates and susceptibility to environmental stress on transplantation. We describe the cellular and molecular characteristics of multilineage-differentiating stress-enduring (Muse) cells derived from adipose tissue (AT), a subpopulation of pluripotent stem cells isolated from human lipoaspirates. Muse-AT cells were efficiently obtained using a simple, fast, and affordable procedure, avoiding cell sorting and genetic manipulation methods. Muse-AT cells isolated under severe cellular stress, expressed pluripotency stem cell markers and spontaneously differentiated into the three germ lineages. Muse-AT cells grown as spheroids have a limited proliferation rate, a diameter of ∼15 µm, and ultrastructural organization similar to that of embryonic stem cells. Muse-AT cells evidenced high stage-specific embryonic antigen-3 (SSEA-3) expression (∼60% of cells) after 7-10 days growing in suspension and did not form teratomas when injected into immunodeficient mice. SSEA-3+ -Muse-AT cells expressed CD105, CD29, CD73, human leukocyte antigen (HLA) class I, CD44, and CD90 and low levels of HLA class II, CD45, and CD34. Using lipopolysaccharide-stimulated macrophages and antigen-challenged T-cell assays, we have shown that Muse-AT cells have anti-inflammatory activities downregulating the secretion of proinflammatory cytokines, such as interferon-γ and tumor necrosis factor-α. Muse-AT cells spontaneously gained transforming growth factor-β1 expression that, in a phosphorylated SMAD2-dependent manner, might prove pivotal in their observed immunoregulatory activity through decreased expression of T-box transcription factor in T cells. Collectively, the present study has demonstrated the feasibility and efficiency of obtaining Muse-AT cells that can potentially be harnessed as immunoregulators to treat immune-related disorders. Stem Cells Translational Medicine 2017;6:161-173.en_US
dc.language.isoenen_US
dc.publisherWiley Open Accessen_US
dc.subjectAntigen-specific responseen_US
dc.subjectImmunomodulationen_US
dc.subjectSpheroids/clustersen_US
dc.subjectStem cellsen_US
dc.titlePluripotent Nontumorigenic Adipose Tissue-Derived Muse Cells have Immunomodulatory Capacity Mediated by Transforming Growth Factor-β1en_US
dc.typeArticleen_US


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